| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Buhrman, Ava L (10)
|
MACVAC |
|
38.08 |
- |
| 2 |
Vercollone, Hartley A (10)
|
MDNBAC |
49.33 |
38.61 |
-10.72 |
| 3 |
Banks, Penelope L (10)
|
MDNBAC |
50.10 |
40.79 |
-9.31 |
| 4 |
Boland, Brooke M (10)
|
MACVAC |
|
41.18 |
- |
| 5 |
Melchior, Ava G (10)
|
MAGPAC |
40.70 |
41.48 |
0.78 |
| 6 |
Anisiobi, Eliza C (10)
|
MDNBAC |
46.18 |
42.09 |
-4.09 |
| 7 |
Peters, Renee E (10)
|
MAWSY |
|
43.35 |
- |
| 8 |
Pukszyn, Elise Q (9)
|
MAEMAC |
41.39 |
44.05 |
2.66 |
| 9 |
Daniel, Roslyn (9)
|
MAWEST |
|
44.25 |
- |
| 10 |
Bender, Isabella R (10)
|
MACVAC |
54.29 |
47.01 |
-7.28 |
| 11 |
Hawthorne, Abigail L (10)
|
MATOPS |
|
49.47 |
- |
| 12 |
Hailu, Wengel (8)
|
MAWEST |
|
50.38 |
- |
| 13 |
Buhrman, Nora L (8)
|
MACVAC |
|
51.34 |
- |
| 14 |
Bender, Adalyn (8)
|
MACVAC |
|
1:14.74 |
- |