| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Millsaps, Katherine G (9)
|
NCYSST |
41.65 |
35.60 |
-6.05 |
| 1 |
Featherston, Alexis J (10)
|
NCLOY |
|
41.16 |
- |
| 1 |
Drozd, Charlotte A (9)
|
NCLOY |
44.98 |
44.40 |
-0.58 |
| 2 |
Cvelic, Summer I (10)
|
NCTYDE |
39.56 |
36.35 |
-3.21 |
| 2 |
Cinson, Alayna M (10)
|
NCYSST |
44.66 |
41.75 |
-2.91 |
| 2 |
Cinson, Evelyn S (9)
|
NCYSST |
57.73 |
47.29 |
-10.44 |
| 3 |
McDonald, Mallory M (9)
|
NCSFSC |
|
39.21 |
- |
| 3 |
Smith, Molly E (10)
|
NCSFSC |
40.64 |
41.98 |
1.34 |
| 3 |
Sechriest, Emory G (10)
|
NCRACY |
1:11.07 |
48.42 |
-22.65 |
| 4 |
McLeod, Ainsley J (10)
|
NCSSS |
|
39.60 |
- |
| 4 |
Ward, Kaylee L (10)
|
NCYBAC |
50.48 |
50.19 |
-0.29 |
| 5 |
McMillan, Emmaleigh R (9)
|
NCYBAC |
55.50 |
51.75 |
-3.75 |
| 6 |
Ward, Maddison L (9)
|
NCYBAC |
50.75 |
52.25 |
1.50 |
| 7 |
Penrod, Nittany J (10)
|
NCSSS |
|
57.29 |
- |