| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Powell, Catherine G (11)
|
NCNCAC |
2:31.04 |
2:28.58 |
-2.46 |
| 2 |
Ghitelman, Susannah K (11)
|
NCNCAC |
2:34.21 |
2:28.87 |
-5.34 |
| 3 |
Hall, Orielle S (11)
|
VANOVA |
2:31.42 |
2:29.39 |
-2.03 |
| 4 |
Duke, Avery T (11)
|
VANOVA |
2:34.61 |
2:30.05 |
-4.56 |
| 5 |
Jacobs, Lane E (11)
|
VANOVA |
2:31.65 |
2:30.83 |
-0.82 |
| 6 |
Wells, Emily M (11)
|
VACA-Y |
2:39.73 |
2:37.24 |
-2.49 |
| 7 |
Fine, Chloe S (11)
|
NCNCAC |
2:38.89 |
2:38.55 |
-0.34 |
| 8 |
Angelopoulou, Sophia M (11)
|
SEBSC |
2:42.74 |
2:38.96 |
-3.78 |
| 9 |
Arms, Madelyn J (11)
|
VAHOKI |
2:56.02 |
2:48.36 |
-7.66 |
| 10 |
Meiferdt, Annalie S (11)
|
NCNCAC |
2:45.09 |
2:49.76 |
4.67 |
| 11 |
FitzGerald, Elizabeth J (11)
|
MDFSC |
3:09.15 |
2:53.44 |
-15.71 |
| 12 |
McCabe, Jillian (11)
|
VAHOKI |
3:01.72 |
2:56.21 |
-5.51 |
| 13 |
Oswald, Avery A (11)
|
VASMAC |
2:57.05 |
3:00.18 |
3.13 |
| 14 |
Pineda, Danna C (11)
|
VALY |
3:16.52 |
3:09.47 |
-7.05 |
| 15 |
Hoke, Audrey E (11)
|
VAHOKI |
3:16.15 |
3:10.31 |
-5.84 |
|
Hamed, Maddie (11)
|
VAHOKI |
|
DQ |
- |
|
Tomlinson, Sophia G (11)
|
MDFSC |
2:44.65 |
DQ |
- |