| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peretti, Samuel B (10)
|
AMWHAT |
38.99 |
37.98 |
-1.01 |
| 2 |
Eckenrode, Jordan K (10)
|
AMSRST |
38.44 |
38.62 |
0.18 |
| 3 |
Kling, Charles P (10)
|
AMSVSC |
41.33 |
43.88 |
2.55 |
| 4 |
Wilson, Lukas A (9)
|
AMRXA |
44.92 |
45.66 |
0.74 |
| 5 |
Roy, Solomon M (10)
|
AMSVSC |
46.46 |
45.84 |
-0.62 |
| 6 |
Sloan, Luke J (10)
|
AMSVSC |
47.71 |
47.23 |
-0.48 |
| 7 |
Eggert, Jaxson J (9)
|
AMWHAT |
48.24 |
49.97 |
1.73 |
| 8 |
Kelly, Scott P (10)
|
AMSVSC |
48.24 |
49.98 |
1.74 |
| 9 |
Weakland, Jackson D (10)
|
AMRXA |
56.48 |
54.81 |
-1.67 |
| 10 |
Schade, Enzo A (9)
|
AMKW |
53.51 |
56.03 |
2.52 |
| 11 |
Blankenship, Tanner A (9)
|
AMCSC |
1:00.37 |
57.46 |
-2.91 |
| 12 |
Sullivan, Parker A (10)
|
AMFAST |
1:05.07 |
58.36 |
-6.71 |
| 13 |
Fuentes da Silva, Luciano M (9)
|
AMFAST |
58.99 |
1:08.09 |
9.10 |
|
Wilson, Dominick K (9)
|
AMWHAT |
1:03.69 |
DQ |
- |
|
Ashead, Zachary J (9)
|
AMSVSC |
46.78 |
DQ |
- |
|
Pirano, Rocco A (10)
|
AMFAST |
58.58 |
DQ |
- |
|
Williams, Noah D (10)
|
AMFAST |
49.83 |
NS |
- |