| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masneri, Olivia C (10)
|
NCGCY |
41.20 |
39.45 |
-1.75 |
| 2 |
Hill, Elizabeth A (10)
|
NCGCY |
44.42 |
43.06 |
-1.36 |
| 3 |
Ross, Sadie B (10)
|
NCGCY |
46.33 |
43.21 |
-3.12 |
| 4 |
Henderson, Brooke C (10)
|
NCGCY |
42.66 |
43.25 |
0.59 |
| 5 |
Kasa, Keira L (9)
|
NCGCY |
46.16 |
44.64 |
-1.52 |
| 6 |
Upton, Camryn M (9)
|
NCGCY |
46.92 |
45.78 |
-1.14 |
| 7 |
Crutchfield, Lucy M (9)
|
NCGCY |
48.01 |
47.39 |
-0.62 |
| 8 |
Todd, Lilly K (9)
|
NCGCY |
53.55 |
49.47 |
-4.08 |
| 9 |
Verdin, Adilyn M (9)
|
NCRACY |
1:08.50 |
56.43 |
-12.07 |
| 10 |
Hawkins, Charlotte J (10)
|
NCRACY |
58.27 |
57.59 |
-0.68 |
| 11 |
Early, Emma E (9)
|
NCGCY |
1:04.84 |
1:00.11 |
-4.73 |
| 12 |
Pearce, Keller E (9)
|
NCGCY |
58.59 |
1:02.04 |
3.45 |
| 13 |
Gaukstern, Josephine M (10)
|
NCRACY |
1:09.73 |
1:10.36 |
0.63 |
|
Alcantara, Ximena (9)
|
NCFAST |
|
DQ |
- |
|
Beuhring, Anna E (9)
|
NCGCY |
53.91 |
DQ |
- |
|
Campbell, Avis D (10)
|
NCGCY |
|
DQ |
- |