| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gabriele, Noah T (16)
|
VAGATR |
49.45 |
48.88 |
-0.57 |
| 2 |
Erb, Zachary J (17)
|
VAGATR |
48.09 |
49.23 |
1.14 |
| 3 |
Dupuis, Aiden J (18)
|
VAGATR |
48.94 |
49.59 |
0.65 |
| 4 |
King, Dylan J (16)
|
VAGATR |
51.52 |
49.63 |
-1.89 |
| 5 |
Cooper, Caleb S (16)
|
VASRVA |
52.04 |
52.97 |
0.93 |
| 6 |
Williams Jr, Kevin N (17)
|
VASRVA |
52.16 |
55.00 |
2.84 |
| 7 |
St. John, William A (17)
|
VAGATR |
53.42 |
55.48 |
2.06 |
| 8 |
Wenger, Isaac S (16)
|
VAGATR |
59.03 |
57.34 |
-1.69 |
| 9 |
Milligan, Nathaniel M (16)
|
VASRVA |
55.76 |
57.41 |
1.65 |
| 10 |
Smith, Marshall W (16)
|
VAGATR |
58.56 |
58.70 |
0.14 |