| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hewitt, Eliza B (10)
|
VAGATR |
31.02 |
31.19 |
0.17 |
| 2 |
Maynard, Addison F (9)
|
VAGATR |
31.18 |
31.68 |
0.50 |
| 3 |
Maust, Claire E (9)
|
VAGATR |
33.69 |
32.67 |
-1.02 |
| 4 |
Baker, Olivia R (10)
|
VAGATR |
34.58 |
35.10 |
0.52 |
| 5 |
Long, Caelee B (9)
|
VAGATR |
36.18 |
35.58 |
-0.60 |
| 6 |
Hitt, Virginia-Annabelle H (9)
|
VASMAC |
35.21 |
39.22 |
4.01 |
| 7 |
Paradise, Anna L (9)
|
VAGATR |
38.88 |
39.97 |
1.09 |
| 8 |
Riner, McKenna J (9)
|
VAGATR |
44.18 |
40.66 |
-3.52 |
| 9 |
Hill, Laci M (10)
|
VAGATR |
38.37 |
41.03 |
2.66 |
| 10 |
Gurth, Madeleine I (10)
|
VAGATR |
|
42.50 |
- |
| 11 |
Toni-Holsinger, Lucy P (9)
|
VAGATR |
51.28 |
45.93 |
-5.35 |
| 12 |
Cacatian, Camille D (9)
|
VASMAC |
46.81 |
48.80 |
1.99 |
| 13 |
Veglio Torres, Paula V (9)
|
VAGATR |
|
1:03.00 |
- |
|
Casterline, Claire F (10)
|
VAGATR |
45.84 |
NS |
- |