| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Olson, Mia V (9)
|
MREAST |
42.73 |
41.13 |
-1.60 |
| 2 |
Fitzpatrick, Emma K (9)
|
MRUN |
48.70 |
45.84 |
-2.86 |
| 3 |
Garcia-Alonso, Alexia (9)
|
MREAST |
47.56 |
46.52 |
-1.04 |
| 4 |
Stone, Sophia K (9)
|
MREAST |
53.72 |
46.74 |
-6.98 |
| 5 |
Kim, Cameron E (9)
|
MREAST |
47.46 |
48.42 |
0.96 |
| 6 |
Wepman, Finley B (9)
|
MREAST |
49.79 |
49.20 |
-0.59 |
| 7 |
Jean-Philippe, Sonali (9)
|
MREAST |
51.67 |
50.59 |
-1.08 |
| 8 |
Mayer, Samara C (9)
|
MREAST |
59.19 |
52.59 |
-6.60 |
| 9 |
Harris, Giana M (9)
|
MREAST |
57.57 |
55.46 |
-2.11 |
| 10 |
Sears, Evelyn M (9)
|
MREAST |
55.41 |
57.40 |
1.99 |