| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Palei, Nicole C (13)
|
MRFREE |
25.48 |
25.47 |
-0.01 |
| 2 |
Esposito, Grace A (13)
|
MRFREE |
27.17 |
26.75 |
-0.42 |
| 3 |
Grandstaff, Julia R (13)
|
MRFREE |
28.53 |
28.18 |
-0.35 |
| 4 |
Goncharuk, Abigail E (13)
|
MRFREE |
30.77 |
29.74 |
-1.03 |
| 5 |
Levitski, Katherina A (13)
|
MRFREE |
30.28 |
30.27 |
-0.01 |
| 6 |
Isakov, Adeline A (13)
|
MRBKR |
29.91 |
30.88 |
0.97 |
| 7 |
Mamot, Elizabeth (13)
|
MRMPNY |
31.37 |
30.90 |
-0.47 |
| 8 |
Sheehan, Maeve E (13)
|
MRFREE |
30.52 |
31.04 |
0.52 |
| 9 |
Gloster, Ella J (13)
|
MRFREE |
39.80 |
32.54 |
-7.26 |
| 10 |
Modeste, Milani S (13)
|
MRBKR |
36.16 |
33.49 |
-2.67 |
| 11 |
Bereznitski, Jane (13)
|
MRMPNY |
33.67 |
33.62 |
-0.05 |
| 12 |
Greenland, Talia P (13)
|
MRBKR |
33.90 |
34.00 |
0.10 |
| 13 |
Seck, Lalia A (13)
|
MRBKR |
34.23 |
34.43 |
0.20 |