| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Esposito, Grace A (13)
|
MRFREE |
58.24 |
56.87 |
-1.37 |
| 1 |
Ramirez, Lily L (13)
|
MRYFD |
58.14 |
58.00 |
-0.14 |
| 2 |
Hoffmann, Vivienne A (13)
|
MRLIFE |
1:03.03 |
1:03.16 |
0.13 |
| 2 |
Bak, Hanna M (13)
|
MRQNS |
58.14 |
58.27 |
0.13 |
| 3 |
Quispe-Justino, Sofia A (13)
|
MRTRI |
1:16.08 |
1:06.34 |
-9.74 |
| 3 |
Brun, Sienna R (13)
|
MRWEST |
58.77 |
58.71 |
-0.06 |
| 4 |
Harkins, Morgan P (13)
|
MRLIE |
59.53 |
59.35 |
-0.18 |
| 5 |
Huang, Cheri K (13)
|
MRFREE |
58.14 |
59.54 |
1.40 |
| 6 |
Wright, Alison E (13)
|
MRYFD |
59.43 |
59.68 |
0.25 |
| 7 |
Akmatbaeva, Deniz (13)
|
MRLGAC |
59.60 |
59.75 |
0.15 |
| 8 |
White, Shannon L (13)
|
MRLIE |
59.49 |
1:00.08 |
0.59 |
| 9 |
Croft, Cecilia C (13)
|
MRYFD |
59.06 |
1:00.10 |
1.04 |
| 10 |
Pagano, Krista R (13)
|
MRLIE |
58.91 |
1:00.20 |
1.29 |
| 11 |
Lam, Kylie L (13)
|
MRQNS |
58.70 |
1:00.66 |
1.96 |
| 12 |
Brcic, Maya (13)
|
MRVIP |
1:01.18 |
1:01.55 |
0.37 |
| 13 |
Chan, Kaylee N (13)
|
MRLIE |
1:00.26 |
1:01.71 |
1.45 |
| 14 |
Tourevski, Sima L (13)
|
MRLIE |
1:01.13 |
1:01.72 |
0.59 |
| 15 |
Karellas, Caroline M (13)
|
MRYFD |
1:01.79 |
1:02.22 |
0.43 |
| 16 |
Masella, Alexandra V (13)
|
MRYFD |
1:01.41 |
1:02.59 |
1.18 |
|
Bennett, Ellen S (13)
|
MRQNS |
1:02.32 |
NS |
- |
|
Anzurez, Olivia (13)
|
MRTRI |
1:06.01 |
NS |
- |