| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Joachim, Gianna M (10)
|
MRNBS |
43.84 |
42.04 |
-1.80 |
| 2 |
Kuzicki, Isabella R (10)
|
MRYBAR |
46.90 |
43.29 |
-3.61 |
| 3 |
McMullan, Hannah R (10)
|
MRBGNW |
44.68 |
43.76 |
-0.92 |
| 4 |
Dominick, Kelcie C (10)
|
MRYMID |
44.44 |
43.81 |
-0.63 |
| 5 |
Fernstrom, Abigail R (10)
|
MRBGNW |
44.58 |
44.32 |
-0.26 |
| 6 |
Santi, Gabriella J (10)
|
MRCOND |
44.81 |
44.73 |
-0.08 |
| 7 |
Kovacs, Adrienn (10)
|
MRAGMV |
46.74 |
44.91 |
-1.83 |
| 8 |
Edelmann, Paige (10)
|
MRCFJV |
46.44 |
45.14 |
-1.30 |
| 9 |
Huber, Lucy R (10)
|
MRNDAC |
44.99 |
45.43 |
0.44 |
| 10 |
Broge, Eleanor H (10)
|
MRWSSC |
46.00 |
45.53 |
-0.47 |
| 11 |
Giarnella, Viviana R (10)
|
MRBGNW |
46.66 |
45.77 |
-0.89 |
| 12 |
la Fleur, Chloe R (10)
|
MRBGNW |
47.62 |
46.10 |
-1.52 |
| 13 |
Kovacs, Alicia (10)
|
MRAGMV |
47.53 |
46.63 |
-0.90 |
|
Ruffy, Rhyanne X (10)
|
MRNYSA |
47.64 |
DQ |
- |
|
Mealli, Ingrid S (10)
|
MRBGNW |
47.55 |
NS |
- |