| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Martinez, Kylie (17)
|
MRHAA |
26.08 |
26.21 |
0.13 |
| 2 |
Mackay, Victoria A (15)
|
MRSSC |
27.13 |
27.05 |
-0.08 |
| 3 |
Vertichio, Kaylie A (17)
|
MRSSC |
26.76 |
27.08 |
0.32 |
| 4 |
Zunich, Daniela (16)
|
MRHAA |
26.30 |
27.11 |
0.81 |
| 5 |
Phillips, Emma G (15)
|
MRHAA |
27.27 |
27.27 |
0.00 |
| 6 |
Cronin, Jessica (16)
|
MRHAA |
26.87 |
27.41 |
0.54 |
| 7 |
McDougall, Marissa R (16)
|
MRCSDC |
27.38 |
27.60 |
0.22 |
| 8 |
Mackay, Alexandria E (17)
|
MRSSC |
28.21 |
28.46 |
0.25 |
| 9 |
Ramos, Dakota (16)
|
MRCSDC |
27.75 |
29.47 |
1.72 |
| 10 |
Fedoriw, Lani (15)
|
MRFA |
31.69 |
29.98 |
-1.71 |
| 11 |
Selvaggio, Theresa L (16)
|
MRSSC |
31.45 |
30.08 |
-1.37 |
| 12 |
Glennon, Abigail K (16)
|
MRSSC |
29.68 |
30.49 |
0.81 |
| 13 |
Nemickas, Eleonora (15)
|
MRCSDC |
32.81 |
30.69 |
-2.12 |
| 14 |
Lewis, Teagan M (15)
|
MRSSC |
31.87 |
31.98 |
0.11 |
| 15 |
Avdoulos, Maggie (15)
|
MRHAA |
32.52 |
32.63 |
0.11 |
| 16 |
Higgins, Keira C (15)
|
MRCSDC |
34.84 |
34.17 |
-0.67 |