| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bonn, Sylvia (10)
|
MRHAA |
35.21 |
36.51 |
1.30 |
| 2 |
Nguyen, Olivia B (10)
|
MRSSC |
34.91 |
36.73 |
1.82 |
| 3 |
Fagundes, Sofia (10)
|
MRHAA |
39.21 |
38.15 |
-1.06 |
| 4 |
Fiore, Julia R (9)
|
MRFA |
44.09 |
41.79 |
-2.30 |
| 5 |
Cantrell, Chloe E (9)
|
MRTVSC |
41.89 |
43.39 |
1.50 |
| 6 |
Fletcher, Reilly D (10)
|
MRFA |
44.81 |
44.41 |
-0.40 |
| 7 |
Villagracia, Madison E (9)
|
MRTVSC |
49.59 |
46.21 |
-3.38 |
| 8 |
Vargas, Izabella C (8)
|
MRTVSC |
43.83 |
46.88 |
3.05 |
| 9 |
Dassen, Arwen M (7)
|
MRTVSC |
46.00 |
48.72 |
2.72 |
| 10 |
Garg, Kinjal (10)
|
MRSSC |
|
51.97 |
- |
| 11 |
DeHart, Addison (9)
|
MRHAA |
1:02.77 |
55.65 |
-7.12 |
| 12 |
Rochlin, Vivienne (8)
|
MRTVSC |
52.15 |
56.21 |
4.06 |
| 13 |
Merenkova, Anastasia (10)
|
MRTVSC |
58.03 |
58.44 |
0.41 |
| 14 |
Patterson, Sophia J (10)
|
MRFA |
|
59.08 |
- |
| 15 |
Chu, Jocelyn C (7)
|
MRTVSC |
1:05.10 |
1:02.11 |
-2.99 |
| 16 |
Kircher, Emma J (9)
|
MRSSC |
|
1:10.75 |
- |
|
Higgins, Faith (10)
|
MRHAA |
1:00.00 |
DQ |
- |