| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Linkonis, Ana V (13)
|
VANOVA |
24.88 |
24.91 |
0.03 |
| 2 |
Jones, Jasper D (13)
|
VANOVA |
25.43 |
24.95 |
-0.48 |
| 3 |
Petronello, Hadley F (13)
|
PVNCAP |
26.22 |
25.11 |
-1.11 |
| 4 |
Gudana, Sophie I (13)
|
PVNCAP |
25.28 |
25.30 |
0.02 |
| 5 |
Willard, Katelyn R (13)
|
VANOVA |
26.09 |
25.44 |
-0.65 |
| 6 |
Schreiber, Kathryn H (13)
|
PVNCAP |
25.77 |
25.78 |
0.01 |
| 7 |
Roemer, Samantha R (13)
|
PVNCAP |
26.16 |
25.84 |
-0.32 |
| 8 |
Karafin, Sasha (13)
|
MRLIAC |
27.61 |
25.98 |
-1.63 |
| 9 |
Dietrich, Stella R (13)
|
VATIDE |
25.51 |
26.07 |
0.56 |
| 10 |
White, Charlotte L (13)
|
VANOVA |
26.48 |
26.31 |
-0.17 |
| 11 |
Gebhard, Madelyn E (13)
|
MRLIAC |
27.47 |
26.93 |
-0.54 |
| 12 |
Davidoff, Sadie C (13)
|
MRLIAC |
28.19 |
26.95 |
-1.24 |
| 13 |
Strach, Piper G (13)
|
VATIDE |
27.01 |
27.13 |
0.12 |
| 14 |
Law, Isabella Y (13)
|
MRLIAC |
27.97 |
27.18 |
-0.79 |
| 15 |
Ervin, Joslyn E (13)
|
VATIDE |
27.24 |
27.22 |
-0.02 |
|
Morrill, Isabella M (13)
|
VATIDE |
25.11 |
DQ |
- |