| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Linkonis, Ana V (14)
|
VANOVA |
24.11 |
24.10 |
-0.01 |
| 2 |
Jones, Jasper D (14)
|
VANOVA |
24.11 |
24.36 |
0.25 |
| 3 |
Gudana, Sophie I (14)
|
PVNCAP |
24.56 |
24.75 |
0.19 |
| 4 |
Roemer, Samantha R (14)
|
PVNCAP |
25.32 |
25.00 |
-0.32 |
| 5 |
Willard, Katelyn R (14)
|
VANOVA |
25.44 |
25.14 |
-0.30 |
| 6 |
Petronello, Hadley F (14)
|
PVNCAP |
25.11 |
25.21 |
0.10 |
| 7 |
Schreiber, Kathryn H (14)
|
PVNCAP |
24.98 |
25.78 |
0.80 |
| 8 |
Patterson, Madelynn A (14)
|
VATIDE |
25.96 |
25.79 |
-0.17 |
| 9 |
Dietrich, Stella R (14)
|
VATIDE |
25.43 |
25.91 |
0.48 |
| 10 |
Peitler, Maya (14)
|
MRLIAC |
26.14 |
26.03 |
-0.11 |
| 11 |
Kryszon, Kylie M (14)
|
VANOVA |
26.28 |
26.54 |
0.26 |
| 12 |
Guzman, Maite A (14)
|
VATIDE |
26.09 |
27.24 |
1.15 |
| 13 |
Beech, Ayla F (14)
|
VATIDE |
31.07 |
30.75 |
-0.32 |
|
Simons, Matthew A (14)
|
MRLIAC |
|
23.84 |
- |
|
Ip, Tyler (14)
|
MRLIAC |
|
24.08 |
- |