| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleming, Brianna M (14)
|
PVNCAP |
2:26.85 |
2:26.08 |
-0.77 |
| 2 |
Shive, Leah W (14)
|
PVNCAP |
2:30.06 |
2:27.11 |
-2.95 |
| 3 |
Dai, Carina Z (14)
|
MRLIAC |
2:31.31 |
2:28.52 |
-2.79 |
| 4 |
Buckley, Olivia L (14)
|
PVNCAP |
2:28.88 |
2:29.38 |
0.50 |
| 5 |
Kryszon, Kendall G (14)
|
VANOVA |
2:30.12 |
2:31.56 |
1.44 |
| 6 |
Linkonis, Ana V (14)
|
VANOVA |
2:25.93 |
2:31.96 |
6.03 |
| 7 |
Roy, Aishwarya S (14)
|
PVNCAP |
2:31.52 |
2:32.87 |
1.35 |
| 8 |
Eberle, Emery F (14)
|
VATIDE |
2:33.69 |
2:35.01 |
1.32 |
| 9 |
Richardson, Reese G (14)
|
VANOVA |
2:34.23 |
2:37.46 |
3.23 |
| 10 |
Karunaratne, Madison A (14)
|
VATIDE |
2:47.81 |
2:45.57 |
-2.24 |
| 11 |
Guzman, Maite A (14)
|
VATIDE |
2:40.27 |
2:47.49 |
7.22 |
| 12 |
Grzybowski, Claire A (14)
|
VATIDE |
2:58.93 |
2:47.60 |
-11.33 |
|
White, Charlotte L (14)
|
VANOVA |
2:26.80 |
DQ |
- |
|
Beech, Ayla F (14)
|
VATIDE |
|
DQ |
- |