| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cutchins, Elizabeth D (12)
|
VATIDE |
2:33.96 |
2:32.33 |
-1.63 |
| 2 |
Klase, Jillian B (12)
|
VANOVA |
2:34.58 |
2:36.97 |
2.39 |
| 3 |
Randsmith, Saxon K (12)
|
PVNCAP |
2:40.49 |
2:38.21 |
-2.28 |
| 4 |
Schreiber, Claire F (12)
|
PVNCAP |
2:42.92 |
2:38.41 |
-4.51 |
| 5 |
Bouchard, Gabrielle M (12)
|
VANOVA |
2:47.82 |
2:40.50 |
-7.32 |
| 6 |
Banks, Hadley (12)
|
VANOVA |
2:45.85 |
2:42.03 |
-3.82 |
| 7 |
Goodloe, Luna S (12)
|
VATIDE |
2:47.09 |
2:43.00 |
-4.09 |
| 8 |
Harms, Stella J (12)
|
PVNCAP |
2:41.76 |
2:44.16 |
2.40 |
| 9 |
Chen, Bella S (12)
|
PVNCAP |
2:46.36 |
2:45.98 |
-0.38 |
| 10 |
Peterson, Campbell M (12)
|
VATIDE |
2:52.32 |
2:50.28 |
-2.04 |
| 11 |
Goins, Rebecca C (12)
|
VANOVA |
2:49.98 |
2:50.47 |
0.49 |
| 12 |
Wu, Georgia M (12)
|
MRLIAC |
3:05.05 |
2:53.06 |
-11.99 |
| 13 |
Gans, Josie L (12)
|
MRLIAC |
3:01.43 |
2:54.56 |
-6.87 |
| 14 |
Shean, May E (12)
|
VATIDE |
3:04.96 |
2:56.87 |
-8.09 |
| 15 |
Hu, Allison (12)
|
MRLIAC |
3:02.22 |
2:58.97 |
-3.25 |