| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bynum, Madeline S (12)
|
VANOVA |
28.05 |
26.71 |
-1.34 |
| 2 |
Harris, Savannah J (12)
|
VANOVA |
28.24 |
28.09 |
-0.15 |
| 3 |
Andrews, Abigail G (12)
|
PVNCAP |
29.82 |
29.52 |
-0.30 |
| 4 |
Cota-Robles, Isabella V (12)
|
PVNCAP |
29.18 |
29.58 |
0.40 |
| 5 |
Gavino, Alessandra B (12)
|
PVNCAP |
29.24 |
29.81 |
0.57 |
| 6 |
Rosade, Elyse M (12)
|
PVNCAP |
29.89 |
29.92 |
0.03 |
| 7 |
Miller, Jayden S (12)
|
VATIDE |
30.38 |
30.11 |
-0.27 |
| 8 |
Lande, Charlotte A (12)
|
VANOVA |
32.55 |
31.06 |
-1.49 |
| 9 |
Simons, Ashley R (12)
|
MRLIAC |
33.41 |
31.24 |
-2.17 |
| 10 |
Lyashenko, Sophia M (12)
|
VATIDE |
32.23 |
31.79 |
-0.44 |
| 11 |
Wilson, Loralei E (12)
|
VATIDE |
33.27 |
31.87 |
-1.40 |
| 12 |
Williams, Caroline H (12)
|
VANOVA |
32.62 |
32.43 |
-0.19 |
|
Perkins, Madison N (12)
|
VATIDE |
33.82 |
NS |
- |