| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moore, Coyle F (12)
|
VATIDE |
25.24 |
25.68 |
0.44 |
| 2 |
O'Neill, Connor F (12)
|
PVNCAP |
27.88 |
26.47 |
-1.41 |
| 3 |
Miklos, Owen T (12)
|
VANOVA |
28.49 |
27.40 |
-1.09 |
| 4 |
Wentland, Phoenix K (12)
|
PVNCAP |
28.99 |
28.35 |
-0.64 |
| 5 |
Van Deren, Jackson R (12)
|
VANOVA |
28.68 |
28.50 |
-0.18 |
| 6 |
Witdoeckt, Josiah D (12)
|
PVNCAP |
28.99 |
28.95 |
-0.04 |
| 7 |
Arcarese, James R (12)
|
VANOVA |
28.96 |
29.34 |
0.38 |
| 8 |
Ellis, Leo T (12)
|
PVNCAP |
33.25 |
29.56 |
-3.69 |
| 9 |
Radoslovich, Steven A (12)
|
MRLIAC |
30.46 |
29.57 |
-0.89 |
| 10 |
Bregman, Nathan R (12)
|
MRLIAC |
31.81 |
29.94 |
-1.87 |
| 11 |
Sardesai, Nolan A (12)
|
VANOVA |
30.38 |
30.20 |
-0.18 |
| 12 |
Wang, Nathan (12)
|
MRLIAC |
32.15 |
31.01 |
-1.14 |
| 13 |
Peng, Warren (12)
|
MRLIAC |
31.75 |
31.41 |
-0.34 |
| 14 |
Taylor, Charlie D (12)
|
VATIDE |
30.08 |
31.43 |
1.35 |
| 15 |
Everhart, Thomas W (12)
|
VATIDE |
33.83 |
32.57 |
-1.26 |
| 16 |
Sanness, Lukas T (12)
|
VATIDE |
34.73 |
34.24 |
-0.49 |