| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Witmer, Gentry L (10)
|
NCNSEA |
43.09 |
42.54 |
-0.55 |
| 2 |
Crowley, Nina L (10)
|
NCNSEA |
43.08 |
43.91 |
0.83 |
| 3 |
Amin, Eliza R (9)
|
NCNSEA |
48.49 |
47.15 |
-1.34 |
| 4 |
Neu, Gina T (10)
|
NCNSEA |
50.55 |
47.39 |
-3.16 |
| 5 |
Blair, Abigail J (10)
|
NCNSEA |
51.46 |
47.94 |
-3.52 |
| 6 |
Petroff, Annderson E (10)
|
NCNSEA |
53.01 |
48.86 |
-4.15 |
| 7 |
Eidson, Aubrey L (10)
|
NCBAC |
|
54.84 |
- |
| 8 |
Hackney, Isabella L (10)
|
NCBAC |
1:12.17 |
1:01.77 |
-10.40 |
| 9 |
Key, Eleanor R (9)
|
NCBAC |
1:09.39 |
1:03.08 |
-6.31 |
| 10 |
Holden Hinton, Helen M (9)
|
NCBAC |
1:08.98 |
1:14.08 |
5.10 |
|
Bascome, Lillian C (9)
|
NCNSEA |
|
DQ |
- |