| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wert, Sophia G (13)
|
NCNSEA |
32.74 |
32.41 |
-0.33 |
| 2 |
Boehmer, Opal G (13)
|
NCBAC |
33.78 |
33.44 |
-0.34 |
| 3 |
Caron, Tess A (14)
|
NCBAC |
34.13 |
33.60 |
-0.53 |
| 4 |
Jackson, Adrianna J (14)
|
NCBAC |
34.03 |
34.21 |
0.18 |
| 5 |
Williams, Ava M (14)
|
NCBAC |
34.41 |
34.26 |
-0.15 |
| 6 |
Long, Abigail G (13)
|
NCBAC |
34.89 |
34.36 |
-0.53 |
| 7 |
Reaves, Camren G (14)
|
NCNSEA |
36.60 |
34.44 |
-2.16 |
| 8 |
McPherson, Brooke C (13)
|
NCBAC |
38.19 |
35.36 |
-2.83 |
| 9 |
Zeron, Finley K (13)
|
NCBAC |
39.35 |
35.91 |
-3.44 |
| 10 |
Jarvis, Kaylee G (14)
|
NCBAC |
49.69 |
36.04 |
-13.65 |
| 11 |
Amin, Lillian M (13)
|
NCNSEA |
35.95 |
36.05 |
0.10 |
| 12 |
Hicks, Addison A (14)
|
NCNSEA |
43.14 |
36.11 |
-7.03 |
| 13 |
Yarnoz, Kemper P (13)
|
NCNSEA |
36.93 |
36.93 |
0.00 |
| 14 |
Crowley, Olivia J (13)
|
NCNSEA |
37.26 |
37.37 |
0.11 |
| 15 |
Key, Abigail O (14)
|
NCBAC |
|
38.84 |
- |
| 16 |
Best, Bella M (13)
|
NCBAC |
54.82 |
43.95 |
-10.87 |
| 17 |
Tomlinson, Maegan I (14)
|
NCBAC |
|
46.16 |
- |
|
Wirszyla, Isabella B (13)
|
NCNSEA |
|
DQ |
- |