| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hronich, Emma A (12)
|
NCSTAR |
28.26 |
27.27 |
-0.99 |
| 2 |
Bumgarner, Carolina F (12)
|
NCSTAR |
28.11 |
28.33 |
0.22 |
| 3 |
Townsend, Abigail C (12)
|
NCSTAR |
29.25 |
28.84 |
-0.41 |
| 4 |
Gribben, Natalie E (11)
|
NCBAQ |
30.42 |
29.92 |
-0.50 |
| 5 |
Balfour, Genevieve J (11)
|
NCSTAR |
30.21 |
30.98 |
0.77 |
| 6 |
Voss, Ava M (11)
|
NCSTAR |
34.82 |
33.22 |
-1.60 |
| 7 |
Fontanella, Joslyn R (11)
|
NCSTAR |
37.08 |
35.62 |
-1.46 |
| 8 |
Wallace, Emory E (12)
|
NCSTAR |
41.52 |
36.96 |
-4.56 |
| 9 |
Jernigan, Clara E (11)
|
NCSTAR |
42.10 |
37.21 |
-4.89 |
| 10 |
Wichman, Charlotte E (11)
|
NCSTAR |
42.98 |
43.67 |
0.69 |
| 11 |
Mann, Caroline M (11)
|
NCSTAR |
|
44.48 |
- |
| 12 |
Mann, Lillian G (11)
|
NCSTAR |
|
45.56 |
- |
|
Kodom, Enynam E (11)
|
NCSTAR |
|
DQ |
- |