| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hopke, Lillian G (10)
|
ADCPP |
28.65 |
29.95 |
1.30 |
| 2 |
Harpootlian, Emma L (9)
|
ADCPP |
34.33 |
35.70 |
1.37 |
| 3 |
Sanghi, Ingrid K (10)
|
ADCPP |
35.59 |
36.47 |
0.88 |
| 4 |
Hopke, Stella M (9)
|
ADCPP |
34.93 |
39.10 |
4.17 |
| 5 |
Hook, Elise C (9)
|
ADCPP |
40.27 |
40.48 |
0.21 |
| 6 |
Armendariz-Huang, Penelope S (10)
|
ADNTS |
43.20 |
42.94 |
-0.26 |
| 7 |
Matt, Keira M (9)
|
ADNHA |
49.75 |
48.22 |
-1.53 |
| 8 |
Gu, Alyssa (9)
|
ADCPP |
49.41 |
48.64 |
-0.77 |
| 9 |
Oldenburgh, Aubrie K (10)
|
ADNTS |
|
50.20 |
- |
| 10 |
Backus, Rory C (10)
|
ADNTS |
|
54.42 |
- |
| 11 |
Bashaw, McKenna M (10)
|
ADNTS |
|
1:04.44 |
- |