| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ryan, Quinlan A (10)
|
ADCPP |
39.33 |
39.10 |
-0.23 |
| 2 |
Hopke, Stella M (9)
|
ADCPP |
42.47 |
41.49 |
-0.98 |
| 3 |
Sanghi, Ingrid K (10)
|
ADCPP |
43.28 |
44.61 |
1.33 |
| 4 |
Hook, Elise C (9)
|
ADCPP |
42.79 |
44.66 |
1.87 |
| 5 |
Armendariz-Huang, Penelope S (10)
|
ADNTS |
50.82 |
49.72 |
-1.10 |
| 6 |
Casalinuovo, Brynn M (10)
|
ADCPP |
47.46 |
50.44 |
2.98 |
| 7 |
Francom, Amelia J (9)
|
ADNTS |
|
53.46 |
- |
| 8 |
WANG, PHIRA Y (9)
|
ADCPP |
58.01 |
55.73 |
-2.28 |
| 9 |
Matt, Keira M (9)
|
ADNHA |
1:00.26 |
57.34 |
-2.92 |
| 10 |
Gu, Alyssa (9)
|
ADCPP |
1:05.20 |
1:03.90 |
-1.30 |
| 11 |
Bashaw, McKenna M (10)
|
ADNTS |
|
1:04.87 |
- |
|
Oldenburgh, Aubrie K (10)
|
ADNTS |
|
DQ |
- |
|
Brencher, Bo E (10)
|
ADNTS |
|
NS |
- |