| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Clara G (9)
|
PVNCAP |
47.19 |
46.70 |
-0.49 |
| 2 |
Reeves, Sachi M (10)
|
PVPAC |
49.94 |
47.01 |
-2.93 |
| 3 |
Cooper, Ava (10)
|
PVPAC |
50.25 |
50.19 |
-0.06 |
| 4 |
Shah, Sena U (9)
|
PVPAC |
52.41 |
53.37 |
0.96 |
| 5 |
Rothleder, Naomi J (9)
|
PVTIBU |
53.90 |
54.68 |
0.78 |
| 6 |
Tofilon, Sophie J (9)
|
PVNCAP |
57.22 |
57.74 |
0.52 |
| 7 |
Zabst, Josephine M (9)
|
PVTIBU |
1:04.12 |
58.97 |
-5.15 |
| 8 |
Yin, Yuxiao (9)
|
PVMSSC |
1:04.23 |
58.98 |
-5.25 |
| 9 |
Cataldo, Gabriella S (10)
|
MDMSC |
|
59.74 |
- |
| 10 |
Spencer, Lois M (10)
|
PVPAC |
57.38 |
59.99 |
2.61 |
| 11 |
Horwitz, Nina R (10)
|
MDMSC |
58.44 |
1:00.37 |
1.93 |
| 12 |
Lopez, Tessa J (9)
|
PVTIBU |
59.30 |
1:00.91 |
1.61 |
| 13 |
Lewis, Harleigh J (9)
|
PVPAC |
1:07.69 |
1:06.59 |
-1.10 |
| 14 |
Kelley, Maya S (10)
|
MDMSC |
1:06.58 |
1:07.71 |
1.13 |
| 15 |
Yin, Lily Y (10)
|
PVMSSC |
|
1:08.18 |
- |
| 16 |
Musok, Mallory S (9)
|
PVTIBU |
|
1:08.30 |
- |
| 17 |
Thakur, Raima (9)
|
PVMSSC |
1:29.06 |
1:10.87 |
-18.19 |
| 18 |
Castonguay, Sarah E (9)
|
PVPAC |
|
1:12.07 |
- |
| 19 |
Ofwono, Faith E (9)
|
PVMSSC |
1:23.82 |
1:12.75 |
-11.07 |
| 20 |
Stepensky, Aleksandra M (9)
|
PVTIBU |
1:15.97 |
1:14.73 |
-1.24 |
| 21 |
Mina, Mackenzie R (9)
|
PVPAC |
1:34.52 |
1:20.41 |
-14.11 |
| 22 |
Yadav, Angel T (9)
|
PVPAC |
|
1:29.53 |
- |
|
Fryer, Janelle G (9)
|
PVPAC |
|
DQ |
- |