| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shive, Keira X (10)
|
PVNCAP |
32.26 |
33.21 |
0.95 |
| 2 |
Reeves, Sachi M (10)
|
PVPAC |
36.46 |
37.17 |
0.71 |
| 3 |
Cooper, Ava (10)
|
PVPAC |
37.30 |
38.24 |
0.94 |
| 4 |
Kidwell, Abbygail A (9)
|
PVPAC |
45.96 |
42.10 |
-3.86 |
| 5 |
Cataldo, Gabriella S (10)
|
MDMSC |
44.86 |
42.99 |
-1.87 |
| 6 |
Zabst, Josephine M (9)
|
PVTIBU |
|
43.35 |
- |
| 7 |
Rothleder, Naomi J (9)
|
PVTIBU |
46.44 |
46.32 |
-0.12 |
| 8 |
Yin, Yuxiao (9)
|
PVMSSC |
47.88 |
46.84 |
-1.04 |
| 9 |
Stepensky, Aleksandra M (9)
|
PVTIBU |
49.27 |
48.82 |
-0.45 |
| 10 |
Sayah, Ava (9)
|
PVASA |
45.75 |
49.24 |
3.49 |
| 11 |
Lewis, Harleigh J (9)
|
PVPAC |
50.34 |
49.64 |
-0.70 |
| 12 |
Yin, Lily Y (10)
|
PVMSSC |
|
50.10 |
- |
| 13 |
Musok, Mallory S (9)
|
PVTIBU |
1:00.04 |
50.48 |
-9.56 |
| 14 |
Mina, Mackenzie R (9)
|
PVPAC |
55.56 |
54.55 |
-1.01 |
| 15 |
Castonguay, Sarah E (9)
|
PVPAC |
52.50 |
54.83 |
2.33 |
| 16 |
Spyrou, Aaron G (9)
|
PVPAC |
58.37 |
57.06 |
-1.31 |
| 17 |
Thakur, Raima (9)
|
PVMSSC |
1:17.17 |
1:00.12 |
-17.05 |
| 18 |
Yadav, Angel T (9)
|
PVPAC |
54.51 |
1:00.49 |
5.98 |
| 19 |
Fryer, Janelle G (9)
|
PVPAC |
1:09.19 |
1:08.18 |
-1.01 |