| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Prompol, Charlotte V (7)
|
PVMACH |
|
38.89 |
- |
| 2 |
Horner, Mckenzie G (7)
|
PVMACH |
39.83 |
39.15 |
-0.68 |
| 3 |
Martin, Rebecca M (7)
|
PVNCAP |
|
40.28 |
- |
| 4 |
Blayney, Kyla R (7)
|
PVMACH |
|
45.44 |
- |
| 5 |
Qin, Zhiyue Z (7)
|
PVNCAP |
48.26 |
47.17 |
-1.09 |
| 6 |
Calway, Harriet M (7)
|
PVMACH |
51.63 |
49.14 |
-2.49 |
| 7 |
Sumrow, Eliza R (7)
|
PVNCAP |
50.71 |
50.07 |
-0.64 |
| 8 |
Jordan, Anna K (7)
|
VATORP |
48.36 |
50.69 |
2.33 |
| 9 |
Campbell-Crawford, Zara A (7)
|
PVNCAP |
48.55 |
50.81 |
2.26 |
| 10 |
Mamillapalli, Ruthvika S (7)
|
PVMACH |
49.98 |
50.95 |
0.97 |
| 11 |
Mello, Molly J (7)
|
PVBWST |
|
51.00 |
- |
| 12 |
Detrisac, Ainsley M (7)
|
PVMACH |
1:09.92 |
54.04 |
-15.88 |
| 13 |
Volovar, Maren G (7)
|
PVMACH |
|
57.23 |
- |
| 14 |
Jaczko, Penrose M (7)
|
PVNCAP |
59.16 |
58.54 |
-0.62 |
| 15 |
Le, Tuyet-Lan (7)
|
PVBWST |
|
1:01.67 |
- |