| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bangert, Anna T (13)
|
PVASA |
28.11 |
28.08 |
-0.03 |
| 2 |
Batty, Kayla M (13)
|
PVPAC |
31.58 |
29.84 |
-1.74 |
| 3 |
Boyd, Adeline A (13)
|
PVDCPR |
31.51 |
30.07 |
-1.44 |
| 4 |
Desel Dolan, Katherine M (14)
|
PVDCPR |
32.82 |
33.14 |
0.32 |
| 5 |
Pyle, Atia P (14)
|
PVDCPR |
|
33.96 |
- |
| 6 |
Zhou, Yina (14)
|
PVDCPR |
37.40 |
36.29 |
-1.11 |
| 7 |
Moore, Avari M (14)
|
PVDCPR |
36.19 |
36.51 |
0.32 |
| 8 |
Archard, Salincya O (13)
|
PVDCPR |
44.96 |
37.26 |
-7.70 |
| 9 |
Lipcon, Sonia A (13)
|
PVPAC |
37.00 |
37.48 |
0.48 |
| 10 |
Stawinski, Alexandra U (13)
|
PVPAC |
43.54 |
46.11 |
2.57 |
|
Guerrieri Brown, Giulia (13)
|
PVDCPR |
35.50 |
NS |
- |