| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horner, Mckenzie G (7)
|
PVMACH |
50.47 |
47.60 |
-2.87 |
| 2 |
Marsh, Sydney V (7)
|
PVASA |
|
51.25 |
- |
| 3 |
Zhao, Ellie (7)
|
PVFISH |
53.51 |
52.23 |
-1.28 |
| 4 |
Allen, Kailyn M (7)
|
PVHACC |
|
52.24 |
- |
| 5 |
Hakimzada, Dunya S (7)
|
PVAAC |
|
52.35 |
- |
| 6 |
Mamillapalli, Ruthvika S (7)
|
PVMACH |
55.83 |
52.56 |
-3.27 |
| 7 |
McCready, Liliana L (7)
|
PVAAC |
|
53.99 |
- |
| 8 |
Wiesbauer, Amelie K (7)
|
PVNCAP |
|
54.06 |
- |
| 9 |
Haddad, Joely A (7)
|
PVASA |
|
55.24 |
- |
| 10 |
Mello, Molly J (7)
|
PVBWST |
|
56.56 |
- |
| 11 |
Krueger, Clementine L (7)
|
PVFISH |
1:04.17 |
56.66 |
-7.51 |
| 12 |
Miller, Reagan C (7)
|
PVNCAP |
1:02.13 |
58.27 |
-3.86 |
| 13 |
Amodio, Gia J (7)
|
PVBWST |
|
58.46 |
- |
| 14 |
Thompson, Mary E (7)
|
PVAAC |
24.97 |
59.04 |
34.07 |
| 15 |
Falsone, Alessandra M (6)
|
PVFISH |
1:16.87 |
59.55 |
-17.32 |
| 16 |
Rodgers, Logan A (7)
|
PVMACH |
|
59.82 |
- |
| 17 |
Suthard, Valerie (7)
|
PVSTJS |
|
1:06.54 |
- |
| 18 |
Patrick, Ellen M (7)
|
PVASA |
|
1:06.90 |
- |
| 19 |
Bricker, Alison J (7)
|
PVFISH |
1:23.95 |
1:09.94 |
-14.01 |
| 20 |
Vela, Diana S (6)
|
PVAAC |
|
1:16.71 |
- |
| 21 |
Henrique, Ava F (7)
|
PVAAC |
|
1:25.92 |
- |
|
Palacio, Isabella (7)
|
PVNCAP |
|
DQ |
- |
|
DeLuca, Noa G (7)
|
PVASA |
|
DQ |
- |
|
Mohr, Sophie-Lou R (6)
|
PVAAC |
|
NS |
- |
|
Lundgren, Anna J (7)
|
PVMACH |
|
NS |
- |