| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Dongen, Emilie M (10)
|
PVSNOW |
33.43 |
32.87 |
-0.56 |
| 2 |
Taphorn, Genevieve F (10)
|
PVFXFX |
37.43 |
36.80 |
-0.63 |
| 3 |
Kollman, Hayden A (10)
|
PVSNOW |
41.07 |
37.20 |
-3.87 |
| 4 |
Don, Audrey H (10)
|
PVPM |
43.57 |
41.09 |
-2.48 |
| 5 |
Meadows, Clara A (10)
|
PVFXFX |
42.04 |
41.44 |
-0.60 |
| 6 |
Kostova, Gabriela H (9)
|
PVPM |
|
44.13 |
- |
| 7 |
Julien, Paige D (10)
|
PVSNOW |
47.14 |
45.43 |
-1.71 |
| 8 |
Brittain, Charlotte R (10)
|
PVAAC |
|
47.21 |
- |
| 9 |
Voltz Puig De La Bel, Alexan (10)
|
PVSNOW |
55.33 |
51.54 |
-3.79 |
| 10 |
Walker, Ava A (8)
|
PVFXFX |
57.54 |
53.06 |
-4.48 |
| 11 |
Choi, Amber G (8)
|
PVMAC |
57.10 |
58.38 |
1.28 |