| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thai, Brandon (10)
|
PVNCAP |
39.19 |
39.54 |
0.35 |
| 2 |
Edwards, Henry L (9)
|
PVMACH |
|
41.23 |
- |
| 3 |
Cherryholmes, Caleb J (10)
|
PVMACH |
|
41.51 |
- |
| 4 |
Beggan, Braxten K (9)
|
PVHACC |
52.48 |
47.64 |
-4.84 |
| 5 |
Okolo, John-Matthew O (9)
|
PVNCAP |
51.31 |
47.77 |
-3.54 |
| 6 |
Tolar, Oliver M (10)
|
PVNCAP |
48.02 |
47.81 |
-0.21 |
| 7 |
Schroeder, Austin (9)
|
PVNCAP |
1:00.00 |
48.32 |
-11.68 |
| 8 |
Acholonu, Arinzechukwu M (9)
|
PVNCAP |
1:00.00 |
49.83 |
-10.17 |
| 9 |
Nguyen, Turner G (8)
|
PVMACH |
1:17.88 |
1:02.85 |
-15.03 |
|
Nguyen, Declan L (10)
|
PVMACH |
41.35 |
DQ |
- |
|
Tutunaru, Mark-Robert (10)
|
PVNCAP |
47.02 |
DQ |
- |
|
DiNicola, Rowan P (10)
|
PVMACH |
|
NS |
- |
|
Liu, Ezekiel G (10)
|
PVNCAP |
50.00 |
NS |
- |
|
McDonald, Ethan S (10)
|
PVNCAP |
43.35 |
NS |
- |
|
Berger, Colby J (9)
|
PVTOLL |
|
NS |
- |