| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masten, Kennedy G (9)
|
PVMACH |
44.98 |
36.33 |
-8.65 |
| 2 |
Wilson, Mara B (10)
|
PVMACH |
50.76 |
37.50 |
-13.26 |
| 3 |
Marm, Reagan E (10)
|
PVNCAP |
|
38.25 |
- |
| 4 |
Emery, Marlo C (10)
|
PVNCAP |
46.89 |
39.21 |
-7.68 |
| 5 |
Pai, Divya I (10)
|
PVMACH |
|
41.85 |
- |
| 6 |
Franklin, Emha R (10)
|
PVMACH |
|
46.50 |
- |
| 7 |
Warren, Vivian G (9)
|
PVMACH |
|
46.61 |
- |
| 8 |
Niebylski, Maia R (10)
|
PVNCAP |
|
48.35 |
- |
| 9 |
Schreiber, Claire F (10)
|
PVNCAP |
|
50.03 |
- |
| 10 |
Frantz, Graycen M (10)
|
PVMACH |
|
1:02.10 |
- |
|
Kibsey, Veronika L (7)
|
PVMACH |
|
DQ |
- |
|
Skeete, Ava R (9)
|
PVPGPR |
|
DQ |
- |
|
Abdoulaye-Brown, Zayvia M (10)
|
PVMACH |
|
NS |
- |
|
Showalter, Elle (10)
|
PVMACH |
|
NS |
- |