| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masten, Kennedy G (9)
|
PVMACH |
41.09 |
32.74 |
-8.35 |
| 2 |
Marm, Reagan E (10)
|
PVNCAP |
|
32.95 |
- |
| 3 |
Emery, Marlo C (10)
|
PVNCAP |
37.76 |
33.20 |
-4.56 |
| 4 |
Lay, Kamryn J (10)
|
PVMACH |
|
35.58 |
- |
| 5 |
Nguyen, Sloane M (8)
|
PVMACH |
40.10 |
36.73 |
-3.37 |
| 6 |
Franklin, Emha R (10)
|
PVMACH |
|
37.07 |
- |
| 7 |
Thomas, Lillian G (9)
|
PVFXFX |
48.09 |
37.80 |
-10.29 |
| 8 |
Warren, Vivian G (9)
|
PVMACH |
45.03 |
37.89 |
-7.14 |
| 9 |
Bravery, Samantha M (8)
|
PVMACH |
40.77 |
38.30 |
-2.47 |
| 10 |
Schreiber, Claire F (10)
|
PVNCAP |
|
38.70 |
- |
| 11 |
Onerci, Gizem (10)
|
PVMACH |
51.72 |
42.18 |
-9.54 |
| 12 |
McLenahan, Chelsea C (9)
|
PVMACH |
|
42.55 |
- |
| 13 |
Curry-Briggs, Sara E (8)
|
PVMACH |
|
44.64 |
- |
| 14 |
Frantz, Graycen M (10)
|
PVMACH |
|
46.78 |
- |
| 15 |
Walker, Ava A (8)
|
PVFXFX |
48.53 |
46.91 |
-1.62 |
| 16 |
Miller, Harper L (7)
|
PVMACH |
|
50.39 |
- |
| 17 |
Detrisac, Ainsley M (6)
|
PVMACH |
|
1:01.95 |
- |
| 18 |
Kibsey, Veronika L (7)
|
PVMACH |
|
1:05.96 |
- |
| 19 |
Yoo, Delaney Z (8)
|
PVMACH |
|
1:07.23 |
- |
|
Herman, June M (8)
|
PVMACH |
|
NS |
- |
|
Pai, Divya I (10)
|
PVMACH |
|
NS |
- |
|
Henger, Amelia J (9)
|
PVNCAP |
|
NS |
- |
|
Abdoulaye-Brown, Zayvia M (10)
|
PVMACH |
|
NS |
- |