| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hayken, Gianna K (12)
|
PVRY |
27.93 |
28.34 |
0.41 |
| 2 |
Elster, Fiona G (12)
|
PVRY |
28.12 |
28.61 |
0.49 |
| 3 |
Leveto, Spencer M (11)
|
PVRY |
30.73 |
29.84 |
-0.89 |
| 4 |
Hendershot, Kelsi A (12)
|
PVPAC |
31.62 |
30.82 |
-0.80 |
| 5 |
Coble, Lily I (12)
|
PVPAC |
31.53 |
31.86 |
0.33 |
| 6 |
Engle, Ellery V (11)
|
PVASA |
32.77 |
32.91 |
0.14 |
| 7 |
Netherton, Scarlett M (11)
|
PVPAC |
31.87 |
33.15 |
1.28 |
| 8 |
Vaughan, Leah S (12)
|
PVPAC |
33.46 |
33.24 |
-0.22 |
| 9 |
Romanowski, Lauren N (11)
|
MDHFY |
30.40 |
33.37 |
2.97 |
| 10 |
Skelly, Alyssa M (12)
|
PVPAC |
35.33 |
33.54 |
-1.79 |
| 11 |
Brown, Leah (12)
|
PVPAC |
46.21 |
54.74 |
8.53 |