| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhang, Emma (6)
|
PVRMSC |
27.02 |
27.62 |
0.60 |
| 2 |
Alsina, Livia (6)
|
PVRMSC |
33.25 |
31.16 |
-2.09 |
| 3 |
Vera, Jubilee K (6)
|
PVFISH |
35.12 |
31.91 |
-3.21 |
| 4 |
White, Stevarra L (5)
|
PVUN |
34.80 |
32.54 |
-2.26 |
| 5 |
Young, Margie (6)
|
PVRMSC |
28.51 |
33.27 |
4.76 |
| 6 |
Luna, Abigail C (6)
|
PVJFD |
36.34 |
34.41 |
-1.93 |
| 7 |
Chang, Colbie (6)
|
PVRMSC |
33.74 |
34.46 |
0.72 |
| 8 |
Liu, Hailey J (6)
|
PVRMSC |
37.11 |
34.85 |
-2.26 |
| 9 |
Ge, Xiyue G (6)
|
PVRMSC |
35.53 |
35.65 |
0.12 |
| 10 |
Nguyen, Hadley B (5)
|
PVFISH |
38.53 |
38.93 |
0.40 |
| 11 |
Qiu, Avery L (6)
|
PVMSSC |
|
43.32 |
- |
| 12 |
Hu, Julia X (6)
|
PVMSSC |
45.28 |
44.09 |
-1.19 |
| 13 |
Bizjak, Maya (6)
|
PVRMSC |
|
44.45 |
- |
|
Bricker, Alison J (6)
|
PVFISH |
|
DQ |
- |
|
Cohen, Gabrielle M (6)
|
PVFISH |
|
DQ |
- |
|
Bruley, Hailey N (6)
|
PVBWST |
40.69 |
DQ |
- |
|
Falsone, Alessandra M (5)
|
PVFISH |
|
NS |
- |