| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roush, Ryleigh J (10)
|
PVSDS |
40.13 |
40.12 |
-0.01 |
| 2 |
Ratner, Emma R (10)
|
PVRIPS |
44.86 |
40.76 |
-4.10 |
| 3 |
Samaras, Susie V (10)
|
PVSDS |
46.93 |
43.39 |
-3.54 |
| 4 |
Kendrick, Hope Q (9)
|
PVSDS |
|
43.44 |
- |
| 5 |
McIlhargie, Quinn B (10)
|
PVSDS |
45.52 |
44.87 |
-0.65 |
| 6 |
Reardon, Alex E (9)
|
PVSDS |
|
45.96 |
- |
| 7 |
Rutherfurd, Sophie M (10)
|
PVSDS |
|
47.73 |
- |
| 8 |
Speight, Joelle E (10)
|
PVSDS |
|
49.05 |
- |
| 9 |
Levine, Lila R (10)
|
PVSDS |
54.09 |
50.17 |
-3.92 |
| 10 |
Shartar, Hadley R (9)
|
PVSDS |
52.85 |
51.82 |
-1.03 |
| 11 |
Atallah, Elise F (9)
|
PVSDS |
49.15 |
52.50 |
3.35 |
| 12 |
Supinger, Veronica L (10)
|
PVSDS |
|
53.53 |
- |
| 13 |
Gordon, Shelby W (10)
|
PVSDS |
|
56.71 |
- |
|
Asaad, Soli J (10)
|
PVSDS |
|
NS |
- |
|
Kerelchuk, Evie R (9)
|
PVSDS |
|
NS |
- |
|
Kerelchuk, Isabel L (9)
|
PVSDS |
|
NS |
- |