| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Oswald, Aiden C (13)
|
VASMAC |
26.25 |
26.23 |
-0.02 |
| 2 |
Hankins, Jackson D (14)
|
VASMAC |
26.95 |
26.78 |
-0.17 |
| 3 |
He, Quinn T (13)
|
VASMAC |
27.61 |
26.89 |
-0.72 |
| 4 |
Yang, Chenghao (13)
|
VAGATR |
27.68 |
27.37 |
-0.31 |
| 5 |
Dmytriyev, Boris (13)
|
VAGATR |
29.26 |
29.42 |
0.16 |
| 6 |
Straight, Christian B (13)
|
VASMAC |
30.03 |
29.53 |
-0.50 |
| 7 |
Dobrynin, Iaroslav (14)
|
VAGATR |
32.04 |
30.83 |
-1.21 |
| 8 |
Dawson, Asher J (13)
|
VAGATR |
39.01 |
30.84 |
-8.17 |
| 9 |
Bucher, Alexander J (13)
|
VAGATR |
|
31.15 |
- |
| 10 |
Kirkpatrick, Brody S (14)
|
VASTRM |
36.58 |
31.57 |
-5.01 |
| 11 |
Fish, Xavier L (13)
|
VASMAC |
29.74 |
32.18 |
2.44 |
| 12 |
Scott, Dylan R (14)
|
VASMAC |
|
32.83 |
- |
| 13 |
Thomas, Caleb P (13)
|
VAGATR |
42.46 |
36.14 |
-6.32 |
| 14 |
Swinson, Levi P (13)
|
VASMAC |
44.27 |
39.05 |
-5.22 |