| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wultsch-Fuller, Sonja E (12)
|
ADNTS |
31.16 |
29.14 |
-2.02 |
| 2 |
Braun, Julia L (11)
|
ADNTS |
28.69 |
29.32 |
0.63 |
| 3 |
Sekelj, Ella S (11)
|
ADNTS |
33.84 |
32.66 |
-1.18 |
| 4 |
Wisniewski, Emma L (12)
|
NIMETS |
|
34.59 |
- |
| 5 |
Heiner, Elizabeth A (11)
|
ADNHA |
40.42 |
38.99 |
-1.43 |
| 6 |
Gioppo, Abigail M (11)
|
ADNHA |
55.35 |
43.34 |
-12.01 |
| 7 |
Andreescu, Alexandra M (11)
|
ADNTS |
|
45.45 |
- |
| 8 |
Bansal, Anya (12)
|
ADNTS |
1:04.14 |
48.00 |
-16.14 |
| 9 |
Fiske, Charlotte M (11)
|
ADNTS |
|
49.73 |
- |
| 10 |
Torres, Andrea (11)
|
ADNTS |
|
51.86 |
- |
| 11 |
Melnikova, Alexandra D (12)
|
ADNTS |
|
52.48 |
- |