| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pfaff, Alison J (16)
|
VAGATR |
26.94 |
27.40 |
0.46 |
| 2 |
Leng, Andrea (18)
|
VAHOKI |
27.26 |
28.25 |
0.99 |
| 3 |
Grafmiller, Madison R (19)
|
VAUN |
28.48 |
28.89 |
0.41 |
| 4 |
Gorby, Tessa L (15)
|
VAGATR |
28.97 |
29.19 |
0.22 |
| 5 |
Bernier, Tattiannah I (14)
|
VAUN-H |
29.73 |
29.29 |
-0.44 |
| 6 |
Grim, Evelyn M (15)
|
VAHOKI |
29.10 |
29.33 |
0.23 |
| 7 |
Durbin, Kathryn A (14)
|
VAHOKI |
29.99 |
30.11 |
0.12 |
| 8 |
Schaaf, Alexis V (13)
|
VAGATR |
30.07 |
30.50 |
0.43 |
| 9 |
Baldwin, Ava S (15)
|
VAHOKI |
30.28 |
30.56 |
0.28 |
| 10 |
Sylvester-Johnson, Jillian B (14)
|
VAGATR |
30.79 |
30.87 |
0.08 |
| 11 |
Russell, Caroline H (17)
|
VALY |
32.48 |
31.06 |
-1.42 |
| 12 |
Gorby, Lila (13)
|
VAGATR |
30.59 |
31.10 |
0.51 |
| 13 |
Linkous, Caroline H (13)
|
VAHOKI |
35.11 |
31.15 |
-3.96 |
| 14 |
Ferreira, Clara (14)
|
VAHOKI |
30.59 |
31.31 |
0.72 |
| 15 |
Byrnes, Jia L (14)
|
VAGATR |
30.60 |
31.69 |
1.09 |
| 16 |
Capelluto, Chiara S (13)
|
VAHOKI |
33.77 |
31.76 |
-2.01 |
| 17 |
Cooper, Amelia C (13)
|
VAGATR |
32.53 |
31.92 |
-0.61 |
| 18 |
Badgley, Annabelle B (16)
|
VAHOKI |
31.55 |
32.05 |
0.50 |
| 19 |
Sanford, Anna M (14)
|
VAHOKI |
31.28 |
32.10 |
0.82 |
| 20 |
Davis, Morgan M (14)
|
VAGATR |
32.14 |
32.19 |
0.05 |
| 21 |
Seidell, Brinna P (14)
|
VAVABR |
34.05 |
32.40 |
-1.65 |
| 22 |
Wohlford, Emma T (14)
|
VAGATR |
32.92 |
32.82 |
-0.10 |
| 23 |
Martin, Hannah C (14)
|
VAHOKI |
31.87 |
33.13 |
1.26 |
| 24 |
Chen, Viena (13)
|
VAHOKI |
41.75 |
34.06 |
-7.69 |
| 25 |
Putallaz, Riley I (14)
|
VAHOKI |
38.15 |
35.02 |
-3.13 |
| 26 |
Dumars, Victoria C (13)
|
VAHOKI |
44.18 |
39.12 |
-5.06 |
|
Bing, Rachael C (14)
|
VAGATR |
31.67 |
NS |
- |
|
Fillo, Grace E (16)
|
VAHOKI |
29.18 |
NS |
- |
|
Muzzy, Ava M (16)
|
VAGATR |
28.05 |
NS |
- |