| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brumback, Noah G (12)
|
NCRSA |
40.30 |
38.79 |
-1.51 |
| 2 |
Nelson, Broderick C (12)
|
VALY |
|
39.10 |
- |
| 3 |
Kohlhofer, Samuel W (12)
|
VAHOKI |
49.35 |
40.69 |
-8.66 |
| 4 |
Giovannini, Niccolo J (12)
|
VAGATR |
|
41.51 |
- |
| 5 |
Handy, Riley J (12)
|
VAVABR |
46.40 |
42.65 |
-3.75 |
| 6 |
Du, Andrew Z (12)
|
VAHOKI |
44.35 |
45.41 |
1.06 |
| 7 |
Parker, Brooks R (12)
|
NCRSA |
50.67 |
47.16 |
-3.51 |
| 8 |
Dmytriyev, Boris (12)
|
VAGATR |
52.84 |
47.84 |
-5.00 |
| 9 |
Thompson, Aspen C (12)
|
VAVABR |
|
47.92 |
- |
| 10 |
Johnson, Alexander M (12)
|
VAGATR |
|
48.83 |
- |
| 11 |
Lawrence, Jack O (12)
|
VAVABR |
|
50.94 |
- |
| 12 |
Bondo, Henry W (12)
|
NCRSA |
51.96 |
51.09 |
-0.87 |