| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Babic, Anastasija P (13)
|
VASRVA |
45.24 |
43.88 |
-1.36 |
| 2 |
Denham, Olivia C (14)
|
VABASS |
41.42 |
44.30 |
2.88 |
| 3 |
Lee, Trinity M (13)
|
VASRVA |
48.75 |
46.70 |
-2.05 |
| 4 |
Rose, Sydney B (13)
|
VASRVA |
46.70 |
46.77 |
0.07 |
| 5 |
Pace, Sophia M (14)
|
VABASS |
58.01 |
47.24 |
-10.77 |
| 6 |
Freid, Blair K (13)
|
VABASS |
40.82 |
47.35 |
6.53 |
| 7 |
Brooks, Madelyne G (14)
|
VABASS |
44.57 |
52.53 |
7.96 |
| 8 |
Griffin, Alexis L (13)
|
VASMAC |
|
52.95 |
- |
| 9 |
Herron, Madeline K (13)
|
VASMAC |
|
1:00.97 |
- |
|
Caten, Margaret M (14)
|
VASRVA |
44.12 |
NS |
- |
|
Henderson, Charlotte L (13)
|
VASRVA |
|
NS |
- |