| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vanyo, Sofie N (13)
|
VACA-Y |
5:17.51 |
5:17.61 |
0.10 |
| 2 |
Heyward, Addison E (14)
|
VA757 |
5:31.24 |
5:26.73 |
-4.51 |
| 3 |
Shnowske, Anna E (14)
|
VA757 |
5:33.14 |
5:27.16 |
-5.98 |
| 4 |
Vernon, Allison L (14)
|
VAQSTS |
5:17.18 |
5:29.29 |
12.11 |
| 5 |
Seay, Madelyn P (13)
|
VAQSTS |
5:28.86 |
5:37.13 |
8.27 |
| 6 |
Czirjak, Sara E (14)
|
VACA-Y |
|
5:39.16 |
- |
| 7 |
Shopbell, Ava M (13)
|
VALY |
6:07.60 |
5:45.09 |
-22.51 |
| 8 |
Wells, Sara T (14)
|
VACA-Y |
5:47.48 |
5:51.64 |
4.16 |
| 9 |
Levin, Isabella G (14)
|
VA757 |
6:03.44 |
5:55.88 |
-7.56 |
| 10 |
Fary, Brenna E (13)
|
VAODAC |
6:06.92 |
5:59.93 |
-6.99 |
| 11 |
Strohhacker, Mackenzie L (14)
|
VAHNVR |
6:15.28 |
6:00.19 |
-15.09 |
| 12 |
Quinn, Brooke A (13)
|
VA757 |
5:58.55 |
6:00.79 |
2.24 |
| 13 |
Hatch, Ruby K (13)
|
VAODAC |
|
6:12.35 |
- |
| 14 |
Grossman, Tate A (14)
|
VACA-Y |
6:31.35 |
6:19.35 |
-12.00 |
| 15 |
Rose, Sydney B (13)
|
VASRVA |
6:40.56 |
6:24.86 |
-15.70 |
| 16 |
Dugger, Sophia A (13)
|
VASRVA |
|
6:25.39 |
- |
| 17 |
Stupar, Aliyah G (14)
|
VACGBD |
|
6:29.03 |
- |
| 18 |
Yancey, Avery E (13)
|
VAODAC |
|
6:33.09 |
- |
| 19 |
Underdown, Keelyn E (13)
|
VAODAC |
|
6:39.80 |
- |
| 20 |
Underdown, Kendall B (13)
|
VAODAC |
|
6:44.58 |
- |
| 21 |
Buyalos, Aubrey J (13)
|
VASRVA |
|
6:53.78 |
- |
|
Dawson, Lina M (14)
|
VAHNVR |
6:18.27 |
NS |
- |
|
Younica, Eleanor A (13)
|
VA757 |
|
NS |
- |