| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trout, Afton A (12)
|
VAHOKI |
38.18 |
38.08 |
-0.10 |
| 2 |
Piechoski, Madison E (11)
|
VAHOKI |
39.24 |
38.52 |
-0.72 |
| 3 |
White, Addison L (10)
|
VAHOKI |
48.91 |
46.04 |
-2.87 |
| 4 |
Burgos, Katie L (10)
|
VAHOKI |
49.03 |
49.23 |
0.20 |
| 5 |
Madsen, Grace E (11)
|
VAHOKI |
|
49.68 |
- |
| 6 |
Hazelwood, Mia G (8)
|
VAHOKI |
52.94 |
50.99 |
-1.95 |
| 7 |
White, William J (7)
|
VAHOKI |
49.52 |
51.76 |
2.24 |
| 8 |
Sanford, Ryan H (8)
|
VAHOKI |
47.45 |
52.55 |
5.10 |
| 9 |
Kuhn, Ella B (9)
|
VAHOKI |
47.48 |
52.56 |
5.08 |
| 10 |
Keister, Jacqueline T (8)
|
VAHOKI |
52.83 |
55.48 |
2.65 |
| 11 |
Harrison, Charlotte E (7)
|
VAHOKI |
1:00.95 |
58.05 |
-2.90 |
| 12 |
Morton, Isabella C (8)
|
VAHOKI |
55.65 |
58.08 |
2.43 |
|
Walsh, Melina G (10)
|
VAUN-H |
|
DQ |
- |