| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bynum, Madeline S (12)
|
VANOVA |
29.57 |
29.29 |
-0.28 |
| 2 |
Causey, Charlotte E (12)
|
VANOVA |
29.96 |
30.58 |
0.62 |
| 3 |
Dee, Tamara Z (12)
|
VANOVA |
33.33 |
32.38 |
-0.95 |
| 4 |
Clark, Peyton J (11)
|
VAPSDN |
33.69 |
32.70 |
-0.99 |
| 5 |
Duke, Avery T (11)
|
VANOVA |
33.70 |
33.40 |
-0.30 |
| 6 |
Porter, Anna J (11)
|
VANOVA |
41.23 |
34.19 |
-7.04 |
| 7 |
Yue, Lindsey W (12)
|
VANOVA |
35.93 |
34.69 |
-1.24 |
| 8 |
Williams, Caroline H (12)
|
VANOVA |
35.17 |
35.21 |
0.04 |
| 9 |
Jung, Olivia M (11)
|
VANOVA |
38.41 |
35.91 |
-2.50 |
| 10 |
Ozlin, Addison L (12)
|
VANOVA |
35.08 |
36.33 |
1.25 |
| 11 |
Gantz, Kara E (12)
|
VANOVA |
35.88 |
36.72 |
0.84 |
| 12 |
Weingartner, Alyssa K (12)
|
VAPSDN |
38.75 |
38.63 |
-0.12 |