| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chethan, Ayan (10)
|
PVNCAP |
40.11 |
39.07 |
-1.04 |
| 2 |
Mollerup, Landon J (10)
|
PVNCAP |
45.53 |
41.17 |
-4.36 |
| 3 |
Cather, Aaron D (10)
|
PVNCAP |
39.59 |
41.23 |
1.64 |
| 4 |
Thai, Brandon (9)
|
PVNCAP |
44.94 |
45.22 |
0.28 |
| 5 |
Corsino, Raine Eli E (10)
|
PVNCAP |
45.38 |
45.45 |
0.07 |
| 6 |
Dockett, Landon S (10)
|
PVNCAP |
50.18 |
47.20 |
-2.98 |
| 7 |
Okolo, John-Matthew O (9)
|
PVNCAP |
49.05 |
47.26 |
-1.79 |
| 8 |
Gaddi, Ryan J (10)
|
PVNCAP |
45.25 |
48.05 |
2.80 |
| 9 |
McDonald, Ethan S (10)
|
PVNCAP |
52.52 |
50.72 |
-1.80 |
| 10 |
Martin, Benjamin R (9)
|
VAVSTP |
52.57 |
50.87 |
-1.70 |
| 11 |
Edwards, Cole M (10)
|
PVNCAP |
51.44 |
51.51 |
0.07 |
| 12 |
Lynch, Gregory D (9)
|
VAVSTP |
52.30 |
56.14 |
3.84 |
| 13 |
Rath, Owen Q (10)
|
PVNCAP |
58.15 |
56.92 |
-1.23 |
|
Silcox, Jonathan T (10)
|
VAVSTP |
|
DQ |
- |