| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harrington, Raleigh P (9)
|
VANOVA |
44.26 |
46.11 |
1.85 |
| 2 |
Gustafson, Daniel L (10)
|
VALY |
47.03 |
47.36 |
0.33 |
| 3 |
Peat, Owen L (10)
|
VANOVA |
48.40 |
47.56 |
-0.84 |
| 4 |
Du, Abraham B (9)
|
VAHOKI |
48.92 |
47.84 |
-1.08 |
| 5 |
Gupton, Samuel B (10)
|
VANOVA |
46.69 |
48.52 |
1.83 |
| 6 |
Tan, Zander (9)
|
VANOVA |
48.75 |
48.93 |
0.18 |
| 7 |
Shiveley, John M (10)
|
VANOVA |
48.98 |
48.98 |
0.00 |
| 8 |
Rhyne, Griffin C (10)
|
NCMOR |
52.75 |
49.19 |
-3.56 |
| 9 |
Worthington, Colston R (10)
|
VANOVA |
49.47 |
50.40 |
0.93 |
| 10 |
Radkevich, Mikhail A (10)
|
VA757 |
51.66 |
50.87 |
-0.79 |
| 11 |
O'Brien, Robert K (9)
|
VA757 |
|
51.38 |
- |
| 12 |
Sunkavalli, Adhvik (10)
|
VANOVA |
53.71 |
52.41 |
-1.30 |
| 13 |
Johnson, Ellis C (10)
|
VANOVA |
56.50 |
52.52 |
-3.98 |
| 14 |
Akesson, Thor E (10)
|
VAPSDN |
|
52.66 |
- |
| 15 |
Hallenborg, Bowen R (9)
|
NCMOR |
50.00 |
52.90 |
2.90 |
| 16 |
Hess, Aaron M (10)
|
VAHOKI |
|
54.23 |
- |
| 17 |
Leger, Noah J (9)
|
VALY |
58.06 |
55.54 |
-2.52 |
| 18 |
Shnowske, Samuel W (10)
|
VA757 |
57.09 |
56.21 |
-0.88 |
| 19 |
Grant, Fischer K (10)
|
VANOVA |
59.31 |
57.00 |
-2.31 |
| 20 |
McMunn, Noah O (10)
|
VAPSDN |
1:03.36 |
59.37 |
-3.99 |
| 21 |
Wolfle, Noah J (9)
|
VANOVA |
56.45 |
59.71 |
3.26 |
| 21 |
Lynch, Gregory D (9)
|
VAVSTP |
1:01.61 |
59.71 |
-1.90 |
| 23 |
Clark, Lucas J (7)
|
VAHOKI |
|
1:01.67 |
- |
| 24 |
McMunn, Eli K (8)
|
VAPSDN |
|
1:04.17 |
- |
| 25 |
Meyer, Aden (8)
|
VAPSDN |
1:08.00 |
1:06.33 |
-1.67 |
| 26 |
Koscielny, Jefferson P (10)
|
VALY |
|
1:10.38 |
- |
| 27 |
McMunn, Max O (8)
|
VAPSDN |
|
1:12.95 |
- |
| 28 |
Holt, Anderson R (8)
|
VALY |
|
1:15.34 |
- |
| 29 |
Givan, Walker R (7)
|
VALY |
|
1:34.95 |
- |
| 30 |
Leger, Benjamin D (6)
|
VALY |
|
1:35.33 |
- |
|
Williams, Benjamin S (6)
|
VAGATR |
1:23.35 |
NS |
- |