| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horst, Callie A (14)
|
VANOVA |
59.89 |
59.83 |
-0.06 |
| 2 |
Callihan, Catherine B (14)
|
VANOVA |
1:03.09 |
1:02.30 |
-0.79 |
| 3 |
Hubbard, Madelyn E (14)
|
VAWAC |
1:03.50 |
1:04.04 |
0.54 |
| 4 |
Hanchett, Kara K (14)
|
VAWAC |
1:04.43 |
1:08.60 |
4.17 |
| 5 |
Phillips, Chloe E (13)
|
VAWAC |
1:09.32 |
1:11.68 |
2.36 |
| 6 |
Townsend, Leah C (13)
|
VAWAC |
1:13.52 |
1:13.72 |
0.20 |
| 7 |
Hoover Smith, Taelyn R (13)
|
VAWAC |
1:25.66 |
1:21.00 |
-4.66 |
| 8 |
Vokes, Jordan E (13)
|
VASTAT |
1:37.60 |
1:30.05 |
-7.55 |
| 9 |
Simone, Olivia G (13)
|
VAWAC |
1:38.76 |
1:31.92 |
-6.84 |
|
Hatzis, Kristina A (14)
|
VASTAT |
1:09.95 |
NS |
- |