| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hopkins, Carroll F (10)
|
MDEST |
43.84 |
43.17 |
-0.67 |
| 2 |
Keimig, Caleb E (10)
|
MDSPRC |
45.21 |
43.60 |
-1.61 |
| 3 |
Li, Daniel V (10)
|
MDEST |
44.47 |
45.95 |
1.48 |
| 4 |
Kondo, Edison S (10)
|
MDSPRC |
51.79 |
48.07 |
-3.72 |
| 5 |
Gilliland, Timothy M (10)
|
MDSPRC |
47.22 |
48.15 |
0.93 |
| 6 |
Faybusovich, Ethan (10)
|
MDEST |
47.27 |
48.20 |
0.93 |
| 7 |
Insignares, Austin V (9)
|
MDEST |
49.74 |
48.77 |
-0.97 |
| 8 |
Cannone, Charles W (10)
|
MDASC |
52.26 |
48.96 |
-3.30 |
| 9 |
Zhou, Joey (10)
|
MDEST |
49.78 |
50.04 |
0.26 |
| 10 |
Hong, Serey R (10)
|
MDCAC |
54.89 |
50.19 |
-4.70 |
| 11 |
McCall, Blake A (10)
|
MDSPRC |
53.58 |
50.95 |
-2.63 |
| 12 |
Sesman, Bryce A (10)
|
MDCAC |
52.78 |
52.43 |
-0.35 |
| 13 |
Kwak, Ethan Y (9)
|
MDEST |
52.39 |
52.48 |
0.09 |
| 14 |
Garvey, Magnus F (10)
|
MDASC |
54.97 |
52.54 |
-2.43 |
| 15 |
Kloster, Cole T (9)
|
MDASC |
52.51 |
53.21 |
0.70 |
| 16 |
Turner, Winston F (9)
|
MDEST |
|
54.27 |
- |
| 17 |
Kalanzi, Enoch A (10)
|
MDASC |
|
54.28 |
- |
| 18 |
Haukebo, James P (9)
|
MDASC |
54.89 |
55.18 |
0.29 |
| 19 |
Hebbel, Henry J (10)
|
MDSPRC |
56.31 |
57.07 |
0.76 |
| 20 |
Chesnokov, Maximilian D (9)
|
MDEST |
57.99 |
57.69 |
-0.30 |
| 21 |
Hawkins, Justin P (9)
|
MDEST |
|
59.97 |
- |
| 22 |
Harrison, Charles A (9)
|
MDSPRC |
57.53 |
1:03.70 |
6.17 |
| 23 |
Shaw, Patrick T (10)
|
MDGBSA |
1:10.23 |
1:07.77 |
-2.46 |
| 24 |
Kodluboy, Carson R (9)
|
MDGMAC |
|
1:08.26 |
- |
| 25 |
Thiel, MacIntyre R (9)
|
MDSPRC |
1:08.24 |
1:08.47 |
0.23 |
| 26 |
Grubb, Liam F (9)
|
MDGBSA |
1:07.74 |
1:12.46 |
4.72 |
| 27 |
Evers, Lukas L (9)
|
MDASC |
|
1:13.68 |
- |
| 28 |
Mott, Nolan M (10)
|
MDGBSA |
|
1:14.04 |
- |
| 29 |
Cashion, Charles D (10)
|
MDASC |
|
1:17.22 |
- |
|
Tom, Holden G (9)
|
MDGBSA |
|
DQ |
- |
|
Houser, Lyndon P (9)
|
MDSPRC |
|
DQ |
- |
|
Kinsey, Joseph A (10)
|
MDASC |
|
DQ |
- |
|
Wang, Daniel Y (9)
|
MDEST |
|
NS |
- |
|
Harper, Julian H (9)
|
MDASC |
58.84 |
NS |
- |