| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masten, Kennedy G (11)
|
PVMACH |
27.33 |
27.46 |
0.13 |
| 2 |
Croghan, Gillan R (11)
|
PVTOLL |
30.97 |
29.92 |
-1.05 |
| 3 |
Han, Riley S (11)
|
PVTOLL |
31.72 |
31.19 |
-0.53 |
| 4 |
Gibson, Kaylee J (11)
|
PVMACH |
34.23 |
32.55 |
-1.68 |
| 5 |
Brooks, Khloe A (11)
|
MDBAY |
33.22 |
32.81 |
-0.41 |
| 6 |
Steber, Alexandra (11)
|
PVMACH |
32.91 |
32.83 |
-0.08 |
| 7 |
Lionel, Caidyn (11)
|
MDBAY |
32.76 |
32.85 |
0.09 |
| 8 |
Plaza, Eva Z (11)
|
PVTOLL |
35.12 |
32.89 |
-2.23 |
| 9 |
Bonderenko, Taylor M (11)
|
PVTOLL |
33.95 |
33.42 |
-0.53 |
| 10 |
Sun, Hope N (11)
|
PVMACH |
35.35 |
33.73 |
-1.62 |
| 11 |
Havrilesky, Melania L (11)
|
PVTOLL |
37.03 |
34.93 |
-2.10 |
| 12 |
Helms, Josephine C (11)
|
MDBAY |
37.78 |
35.52 |
-2.26 |
| 13 |
Paisley, Sydney Q (11)
|
PVMACH |
35.25 |
35.79 |
0.54 |
| 14 |
In, Bella (11)
|
PVMACH |
38.54 |
36.15 |
-2.39 |
| 15 |
Meyers, Clara E (11)
|
PVMACH |
37.33 |
36.43 |
-0.90 |
| 16 |
Laurenzi, Annabelle W (11)
|
PVTOLL |
38.27 |
36.55 |
-1.72 |
| 17 |
Long, Caelee B (11)
|
VAGATR |
34.74 |
36.69 |
1.95 |
| 18 |
Washko, Lillian (11)
|
VAHVAC |
|
45.31 |
- |
|
Coulter, Katherine A (11)
|
VAGATR |
34.74 |
NS |
- |