| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cooper, Jackson R (16)
|
MREAST |
49.46 |
50.52 |
1.06 |
| 2 |
Graber, Seth M (16)
|
MREAST |
50.67 |
51.62 |
0.95 |
| 3 |
Koenigsberg, Samuel J (15)
|
MREAST |
51.68 |
53.60 |
1.92 |
| 4 |
Rutishauser, Wyatt W (18)
|
MREAST |
50.61 |
54.75 |
4.14 |
| 5 |
Kiyici, Koray (15)
|
MREAST |
54.89 |
55.39 |
0.50 |
| 6 |
Boyer, Jackson X (15)
|
MREAST |
53.90 |
56.08 |
2.18 |
| 8 |
Gonzalez, Demetri (15)
|
MREAST |
58.69 |
58.62 |
-0.07 |
| 9 |
Papaioannou, Georgios F (15)
|
CTGRYM |
1:03.50 |
1:02.08 |
-1.42 |
| 10 |
Amen, Logan B (12)
|
CTGRYM |
|
1:17.34 |
- |
| 11 |
Bandsma, Oran I (11)
|
MREAST |
|
1:34.70 |
- |